DSpace Collection: scholarly

Identification of GyrA mutations conferring fluoroquinolone resistance in Salmonella isolated from poultry and swine from Ogun and Oyo State, Nigeria

2011-01-01T00:00:00Z

Title: Identification of GyrA mutations conferring fluoroquinolone resistance in Salmonella isolated from poultry and swine from Ogun and Oyo State, Nigeria Authors: Ogunleye, A.O.; Ajuwape, A.T.P.; Adetosoye, A.I.; Carlson, S.A. Abstract: The quinolone-resistancc determining-region of gyrA was PCR-amplified and sequenced in eight fluoroquinolone-resistant Salmonella (lcvofloxacin MICs ranging from 32-64 pg /tnL) isolated from apparently healthy swine (n=2 isolates) and poultry (n=6 isolates) that died of septicacmic clinical diseases in Ogun and Oyo State, Nigeria. All of the eight isolates possessed the gyrA mutation encoding the histidine=>tyrosine conversion at amino acid 150 (150His=>Tyr). Additional substitutions included: 83Tyr=>Ser in one Salmonella enterica serotype Give isolate (pig), 87Asp=>Tyr in one Salmonella enterica serotype Kentucky isolate (pig); 83Tyr=>Phe in one isolate characterized as 9,12:Nonmotile (poultry); and 87Asp=>Gly in three poultry' isolates including Salmonella enterica serotypes Kentucky (n=2), and Ituri (n=l). The 150His=>Tyr is novel while the other mutations have been previously reported in Salmonella spp. This is the first study to associate gyrA mutations with fluoroquinolone resistance in Nigeria, where fluoroquinolone use in livestock is not tightly regulated Description: In: O. Akinloye, Biotechnology:Trends in Advancement of Life Science Research and Development in Nigeria. Pp. 127 - 131

Small bowel responses to enteral honey and glutamine administration following massive small bowel resection in rabbit

2008-01-01T00:00:00Z

Title: Small bowel responses to enteral honey and glutamine administration following massive small bowel resection in rabbit Authors: Eyarefe, O.D.; Emikpe, B. O.; Arowolo, O. A. Abstract: The trophic effects of honey and glutamine in the healing and adaptation of the small bowel following intestinal resection were studied in some Nigerian non-descript breeds of rabbits. Nine rabbits of mixed sexes with mean body weight of 1.45 ±0.55kg were used. They were randomised into three treatment groups following 50% small bowel resection. Group A rabbits were placed on oral honey treatment. Group B on oral glutamine and group C on normal saline (control). All groups exhibited signs of small bowel adaptation (glutamine(B) honey (A) control (C) at the end of the experiment (4 weeks) with oral glutamine showing the best overall effects on intestinal mucosal growth and adaptation evidenced by significant increase (P<0.05) in residual bow'el length (37.3%), villi width (20.0%), crypt depth (113.3%) and a non significant increase in villus height (33.3%) and cellular mass (10.2%). Honey showed a better effect than control with a significant increase (P<0.05) in villi width (18.2%), crypts depth (66.7%) and cellular mass (33.9%) and a non significant increase in gross residual bow'el length (24.6%), and villus height (30.5%). Our result shows that honey and glutamine have trophic effects on bowel mucosa healing and hyperplasia and have potential therapeutic effects on massive bowel resection in humans

Splenic lymphoma in an adult local bitch-a case report

2008-01-01T00:00:00Z

Title: Splenic lymphoma in an adult local bitch-a case report Authors: Oni, S. O.; Akinrinmade, J. F.; Ajadi, R. A.; Eyarefe, O.D.; Olaifa, A. K. Abstract: A four-year old local dog weighing 12kg was presented four days after signs of persistent anorexia and dullness were observed. Physical examination of the bitch revealed a circumscribed distension of the left lateral abdomen about 5cm caudal to the rib cage, which on palpation appeared firm, discrete and nodular but painless. X-rays views confirmed that the mass was intrasplenic and had a fat density. Blood and urine samples were analysed, the result of which showed proteinuria, bilirubinuria and neutrophilic leucocyosis. Exploratory laparotomy and spleenectomy were carried out. The extirpated spleen had pendunculated projection. The biopsy of the spleen revealed that the tumour is a lymphoma

Surgical asepsis

2014-01-01T00:00:00Z

Title: Surgical asepsis Authors: Eyarefe, O.D. Description: In: A. Adetunji, Essential veterinary surgery, Ibadan University press. 2014. Pp. 197 - 210