Please use this identifier to cite or link to this item:
Title: Polymerase chain reaction assay of ureaplasma strains isolated from high vaginal swabs of women in Ibadan, Nigeria
Authors: Agbakoba, N. R.
Adetosoye, A. I.
Adesina, O. A.
Adewole, I. F.
Keywords: Ureaplasma urealyticum
Polymerase chain reaction
Issue Date: 2008
Publisher: College of Medicine, University of Ibadan, Ibadan
Abstract: Human ureaplasma previously had one species known as Ureaplasma urealyticum but was recently separated into 2 species, U. urealyticum and U. parvum. This study was carried out to separate the ureaplasma strains isolated from women attending a tertiary-care hospital in Nigeria. Thirty (30) Ureaplasma strains isolated from the vaginal tracts of 13 pregnant and 17 non-pregnant women were assayed. The polymerase chain reaction (PCR) technique was employed using two primer pairs: UMS-170/UMA-263 specific for U. urealyticum and UMS-57/UMA-222 specific for U. parvum. The positivity bands of the primer pairs were 476 bp and 326 bp for U. urealyticum and U. parvum respectively. All isolates were found to be U. urealyticumn (100%). Eleven (84.6%) of the 13 U. urealyticum from pregnant women were from asymptomatic women while from the non-pregnant women; 6 (35.3%) were from women with complaint of infertility problems; 5 (29.4%) from those who complained of vaginal discharge, one (5.9%) was asymptomatic while the remaining 5 (29.4%) had various other complaints. U. urealyticum is thus the prevalent species of Ureaplasma among pregnant and non-pregnant women in the study population and this to the best of our knowledge is a pioneer study to speciate human ureaplasmas in this country.
ISSN: 0309-3913
Appears in Collections:scholarly works

Files in This Item:
File Description SizeFormat 
(21) ui_art_agbakoba_polymerase_2008.pdf3.83 MBAdobe PDFThumbnail

Items in UISpace are protected by copyright, with all rights reserved, unless otherwise indicated.